Functional Analysis of Male Mouse Haploid Germ Cells of Various Differentiation Stages: Early and Late Round Spermatids Are Functionally Equivalent in Producing Progeny1

Functional Concentrations of BMP4 on Differentiation of Mouse Embryonic Stem Cells to Primordial Germ Cells

P-50: Elongating and Elongated Spermatids Manufactured In Vitro from Non-Human Primate Pluripotent Stem Cells

Background: We have recently shown that human embryonic (hESCs) and induced pluripotent stem cells (hiPSCs) can differentiate into advanced spermatogenic cells including round spermatids by in vitro culture (Easley et al., Direct differentiation of human pluripotent stem cells into haploid spermatogenic cells. Cell Reports 2, 440-446 2012) and also, in collaboration, that rhesus spermatogonial ...

O-9: Generation of Haploid Spermatids with Fertilization and Development Capacity from Human Spermatogonial Stem Cells of Cryptorchid Patients

Background Infertility affects around 15% of couples, and male factors account for 50%. Cryptorchidism is one of the most common causes for azoospermia. Generation of functional spermatids from azoospermia patients is of unusual significance for treating male infertility. It has been recently reported by peers and us that human spermatogonial stem cells (SSCs) can be clearly identified and cult...

Full-term development of offspring using round spermatids produced ectopically from fetal male germ cells.

The continuous production of mammalian sperm is maintained by the proliferation and differentiation of spermatogonial stem cells, which originate from primordial germ cells in the early embryo. Previously, we reported that the transplantation of fetal male gonadal tissue into the recipient testis was effective obtaining functional sperm. This transplantation technique is a promising new approac...

Behaviour of germ cells and sexual differentiation in late embryonic and early postnatal mouse chimeras.

The purpose of the present study was to trace the fate of primordial germ cells in mouse chimeras of XX/XY constitution. In this type of research one hopes to obtain knowledge of the role of intrinsic genetic factors and of environmental factors (the environment provided by the gonads) in initiating and directing the course of gametogenesis in mammals. Data obtained up to the present show that ...